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Characterization of Immobilized Thermostable Alkaline Protease 50a using Calcium Alginate
Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
*Email Address : n_azlina@umk.edu.my
Abstract : Thermostable alkaline protease 50a (TAP50a) enzyme has been immobilized by calcium alginate due to maintain its characterization. It is done to get better potential in its use when applied and mixed with other ingredients. Alkaline protease immobilization play an important role in detergent industry to facilitate the discharge of super molecule materials in stains like those of milk and blood in the better ways. TAP50a were immobilized with 2%-4% (w/w) of sodium alginate solution to get the best sodium alginate concentration. The optimal immobilization conditions of TAP50a in calcium alginate beads were investigated and obtained with 3% (w/w) alginate using TAP50a (10 U/ml) and 0.1 M CaCl2 solutions. The hydrolytic properties of free TAP50a and immobilized TAP50a in terms of stability as well as the effects of the temperatures and metal ions were determined. The optimum activity of both the free TAP50a and the immobilized TAP50a was at 90°C. Hence, we propose that, this method can be used to produce immobilized TAP50a which can be used in various areas such as detergent additives and dehairing hides.
Keywords : Protease; immobilization; thermostability, calcium alginate